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vcfconcat.c
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/* vcfconcat.c -- Concatenate or combine VCF/BCF files.
Copyright (C) 2013-2014 Genome Research Ltd.
Author: Petr Danecek <[email protected]>
Permission is hereby granted, free of charge, to any person obtaining a copy
of this software and associated documentation files (the "Software"), to deal
in the Software without restriction, including without limitation the rights
to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
copies of the Software, and to permit persons to whom the Software is
furnished to do so, subject to the following conditions:
The above copyright notice and this permission notice shall be included in
all copies or substantial portions of the Software.
THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
THE SOFTWARE. */
#include <stdio.h>
#include <unistd.h>
#include <getopt.h>
#include <string.h>
#include <errno.h>
#include <math.h>
#include <htslib/vcf.h>
#include <htslib/synced_bcf_reader.h>
#include <htslib/kseq.h>
#include "bcftools.h"
typedef struct _args_t
{
bcf_srs_t *files;
htsFile *out_fh;
int output_type;
bcf_hdr_t *out_hdr;
int *seen_seq;
// phasing
int *start_pos, start_tid, ifname;
int *swap_phase, nswap, *nmatch, *nmism;
bcf1_t **buf;
int nbuf, mbuf, prev_chr, min_PQ, prev_pos_check;
int32_t *GTa, *GTb, mGTa, mGTb, *phase_qual, *phase_set;
char **argv, *output_fname, *file_list, **fnames, *regions_list;
int argc, nfnames, allow_overlaps, phased_concat, remove_dups, regions_is_file;
}
args_t;
static void init_data(args_t *args)
{
bcf1_t *line = NULL;
// With phased concat, the chunks overlap and come in the right order. To
// avoid opening all files at once, store start positions to recognise need
// for the next one. This way we can keep only two open chunks at once.
if ( args->phased_concat )
{
args->start_pos = (int*) malloc(sizeof(int)*args->nfnames);
line = bcf_init();
}
kstring_t str = {0,0,0};
int i, prev_chrid = -1;
for (i=0; i<args->nfnames; i++)
{
htsFile *fp = hts_open(args->fnames[i], "r"); if ( !fp ) error("Failed to open: %s\n", args->fnames[i]);
bcf_hdr_t *hdr = bcf_hdr_read(fp); if ( !hdr ) error("Failed to parse header: %s\n", args->fnames[i]);
if ( !args->out_hdr )
args->out_hdr = bcf_hdr_dup(hdr);
else
{
bcf_hdr_combine(args->out_hdr, hdr);
if ( bcf_hdr_nsamples(hdr) != bcf_hdr_nsamples(args->out_hdr) )
error("Different number of samples in %s. Perhaps \"bcftools merge\" is what you are looking for?\n", args->fnames[i]);
int j;
for (j=0; j<bcf_hdr_nsamples(hdr); j++)
if ( strcmp(args->out_hdr->samples[j],hdr->samples[j]) )
error("Different sample names in %s. Perhaps \"bcftools merge\" is what you are looking for?\n", args->fnames[i]);
}
if ( args->phased_concat )
{
int ret = bcf_read(fp, hdr, line);
if ( ret!=0 ) args->start_pos[i] = -2; // empty file
else
{
int chrid = bcf_hdr_id2int(args->out_hdr,BCF_DT_CTG,bcf_seqname(hdr,line));
args->start_pos[i] = chrid==prev_chrid ? line->pos : -1;
prev_chrid = chrid;
}
}
bcf_hdr_destroy(hdr);
hts_close(fp);
}
free(str.s);
if ( line ) bcf_destroy(line);
args->seen_seq = (int*) calloc(args->out_hdr->n[BCF_DT_CTG],sizeof(int));
if ( args->phased_concat )
{
bcf_hdr_append(args->out_hdr,"##FORMAT=<ID=PQ,Number=1,Type=Integer,Description=\"Phasing Quality (bigger is better)\">");
bcf_hdr_append(args->out_hdr,"##FORMAT=<ID=PS,Number=1,Type=Integer,Description=\"Phase Set\">");
}
bcf_hdr_append_version(args->out_hdr, args->argc, args->argv, "bcftools_concat");
args->out_fh = hts_open(args->output_fname,hts_bcf_wmode(args->output_type));
if ( args->out_fh == NULL ) error("Can't write to \"%s\": %s\n", args->output_fname, strerror(errno));
bcf_hdr_write(args->out_fh, args->out_hdr);
if ( args->allow_overlaps )
{
args->files = bcf_sr_init();
args->files->require_index = 1;
if ( args->regions_list )
{
if ( bcf_sr_set_regions(args->files, args->regions_list, args->regions_is_file)<0 )
error("Failed to read the regions: %s\n", args->regions_list);
}
for (i=0; i<args->nfnames; i++)
if ( !bcf_sr_add_reader(args->files,args->fnames[i]) ) error("Failed to open %s: %s\n", args->fnames[i],bcf_sr_strerror(args->files->errnum));
}
else if ( args->phased_concat )
{
// Remove empty files from the list
int nok = 0;
while (1)
{
while ( nok<args->nfnames && args->start_pos[nok]!=-2 ) nok++;
if ( nok==args->nfnames ) break;
i = nok;
while ( i<args->nfnames && args->start_pos[i]==-2 ) i++;
if ( i==args->nfnames ) break;
int tmp = args->start_pos[nok]; args->start_pos[nok] = args->start_pos[i]; args->start_pos[i] = tmp;
char *str = args->fnames[nok]; args->fnames[nok] = args->fnames[i]; args->fnames[i] = str;
}
for (i=nok; i<args->nfnames; i++) free(args->fnames[i]);
args->nfnames = nok;
for (i=1; i<args->nfnames; i++)
if ( args->start_pos[i-1]!=-1 && args->start_pos[i]!=-1 && args->start_pos[i]<args->start_pos[i-1] )
error("The files not in ascending order: %d in %s, %d in %s\n", args->start_pos[i-1]+1,args->fnames[i-1],args->start_pos[i]+1,args->fnames[i]);
args->prev_chr = -1;
args->swap_phase = (int*) calloc(bcf_hdr_nsamples(args->out_hdr),sizeof(int));
args->nmatch = (int*) calloc(bcf_hdr_nsamples(args->out_hdr),sizeof(int));
args->nmism = (int*) calloc(bcf_hdr_nsamples(args->out_hdr),sizeof(int));
args->phase_qual = (int32_t*) malloc(bcf_hdr_nsamples(args->out_hdr)*sizeof(int32_t));
args->phase_set = (int32_t*) malloc(bcf_hdr_nsamples(args->out_hdr)*sizeof(int32_t));
args->files = bcf_sr_init();
args->files->require_index = 1;
args->ifname = 0;
}
}
static void destroy_data(args_t *args)
{
int i;
for (i=0; i<args->nfnames; i++) free(args->fnames[i]);
free(args->fnames);
if ( args->files ) bcf_sr_destroy(args->files);
if ( hts_close(args->out_fh)!=0 ) error("hts_close error\n");
bcf_hdr_destroy(args->out_hdr);
free(args->seen_seq);
free(args->start_pos);
free(args->swap_phase);
for (i=0; i<args->mbuf; i++) bcf_destroy(args->buf[i]);
free(args->buf);
free(args->GTa);
free(args->GTb);
free(args->nmatch);
free(args->nmism);
free(args->phase_qual);
free(args->phase_set);
}
int vcf_write_line(htsFile *fp, kstring_t *line);
#define SWAP(type_t, a, b) { type_t t = a; a = b; b = t; }
static void phase_update(args_t *args, bcf_hdr_t *hdr, bcf1_t *rec)
{
int i, nGTs = bcf_get_genotypes(hdr, rec, &args->GTa, &args->mGTa);
for (i=0; i<bcf_hdr_nsamples(hdr); i++)
{
if ( !args->swap_phase[i] ) continue;
int *gt = &args->GTa[i*2];
if ( bcf_gt_is_missing(gt[0]) || gt[1]==bcf_int32_vector_end ) continue;
SWAP(int, gt[0], gt[1]);
gt[1] |= 1;
}
bcf_update_genotypes(hdr,rec,args->GTa,nGTs);
}
static void phased_flush(args_t *args)
{
if ( !args->nbuf ) return;
bcf_hdr_t *ahdr = args->files->readers[0].header;
bcf_hdr_t *bhdr = args->files->readers[1].header;
int i, j, nsmpl = bcf_hdr_nsamples(args->out_hdr);
for (i=0; i<args->nbuf; i+=2)
{
bcf1_t *arec = args->buf[i];
bcf1_t *brec = args->buf[i+1];
int nGTs = bcf_get_genotypes(ahdr, arec, &args->GTa, &args->mGTa);
if ( nGTs < 0 ) error("GT is not present at %s:%d\n", bcf_seqname(ahdr,arec), arec->pos+1);
if ( nGTs != 2*nsmpl ) continue; // not diploid
nGTs = bcf_get_genotypes(bhdr, brec, &args->GTb, &args->mGTb);
if ( nGTs < 0 ) error("GT is not present at %s:%d\n", bcf_seqname(bhdr,brec), brec->pos+1);
if ( nGTs != 2*nsmpl ) continue; // not diploid
for (j=0; j<nsmpl; j++)
{
int *gta = &args->GTa[j*2];
int *gtb = &args->GTb[j*2];
if ( gta[1]==bcf_int32_vector_end || gtb[1]==bcf_int32_vector_end ) continue;
if ( bcf_gt_is_missing(gta[0]) || bcf_gt_is_missing(gta[1]) || bcf_gt_is_missing(gtb[0]) || bcf_gt_is_missing(gtb[1]) ) continue;
if ( !bcf_gt_is_phased(gta[1]) || !bcf_gt_is_phased(gtb[1]) ) continue;
if ( bcf_gt_allele(gta[0])==bcf_gt_allele(gta[1]) || bcf_gt_allele(gtb[0])==bcf_gt_allele(gtb[1]) ) continue;
if ( bcf_gt_allele(gta[0])==bcf_gt_allele(gtb[0]) && bcf_gt_allele(gta[1])==bcf_gt_allele(gtb[1]) )
{
if ( args->swap_phase[j] ) args->nmism[j]++; else args->nmatch[j]++;
}
if ( bcf_gt_allele(gta[0])==bcf_gt_allele(gtb[1]) && bcf_gt_allele(gta[1])==bcf_gt_allele(gtb[0]) )
{
if ( args->swap_phase[j] ) args->nmatch[j]++; else args->nmism[j]++;
}
}
}
for (i=0; i<args->nbuf/2; i+=2)
{
bcf1_t *arec = args->buf[i];
bcf_translate(args->out_hdr, args->files->readers[0].header, arec);
if ( args->nswap )
phase_update(args, args->out_hdr, arec);
bcf_update_format_int32(args->out_hdr,arec,"PS",args->phase_set,nsmpl);
bcf_write(args->out_fh, args->out_hdr, arec);
if ( arec->pos < args->prev_pos_check ) error("FIXME, disorder: %s:%d vs %d [1]\n", bcf_seqname(args->files->readers[0].header,arec),arec->pos+1,args->prev_pos_check+1);
args->prev_pos_check = arec->pos;
}
args->nswap = 0;
for (j=0; j<nsmpl; j++)
{
if ( args->nmatch[j] >= args->nmism[j] )
args->swap_phase[j] = 0;
else
{
args->swap_phase[j] = 1;
args->nswap++;
}
if ( args->nmatch[j] && args->nmism[j] )
{
// Entropy-inspired quality. The factor 0.7 shifts and scales to (0,1)
double f = (double)args->nmatch[j]/(args->nmatch[j]+args->nmism[j]);
args->phase_qual[j] = 99*(0.7 + f*log(f) + (1-f)*log(1-f))/0.7;
}
else
args->phase_qual[j] = 99;
args->nmatch[j] = 0;
args->nmism[j] = 0;
}
int PQ_printed = 0;
for (; i<args->nbuf; i+=2)
{
bcf1_t *brec = args->buf[i+1];
bcf_translate(args->out_hdr, args->files->readers[1].header, brec);
if ( !PQ_printed )
{
bcf_update_format_int32(args->out_hdr,brec,"PQ",args->phase_qual,nsmpl);
PQ_printed = 1;
for (j=0; j<nsmpl; j++)
if ( args->phase_qual[j] < args->min_PQ ) args->phase_set[j] = brec->pos+1;
}
if ( args->nswap )
phase_update(args, args->out_hdr, brec);
bcf_update_format_int32(args->out_hdr,brec,"PS",args->phase_set,nsmpl);
bcf_write(args->out_fh, args->out_hdr, brec);
if ( brec->pos < args->prev_pos_check ) error("FIXME, disorder: %s:%d vs %d [2]\n", bcf_seqname(args->files->readers[1].header,brec),brec->pos+1,args->prev_pos_check+1);
args->prev_pos_check = brec->pos;
}
args->nbuf = 0;
}
static void phased_push(args_t *args, bcf1_t *arec, bcf1_t *brec)
{
if ( arec && arec->errcode )
error("Parse error at %s:%d, cannot proceed: %s\n", bcf_seqname(args->files->readers[0].header,arec),arec->pos+1, args->files->readers[0].fname);
if ( brec && brec->errcode )
error("Parse error at %s:%d, cannot proceed: %s\n", bcf_seqname(args->files->readers[1].header,brec),brec->pos+1, args->files->readers[1].fname);
int i, nsmpl = bcf_hdr_nsamples(args->out_hdr);
int chr_id = bcf_hdr_name2id(args->out_hdr, bcf_seqname(args->files->readers[0].header,arec));
if ( args->prev_chr<0 || args->prev_chr!=chr_id )
{
if ( args->prev_chr>=0 ) phased_flush(args);
for (i=0; i<nsmpl; i++)
args->phase_set[i] = arec->pos+1;
if ( args->seen_seq[chr_id] ) error("The chromosome block %s is not contiguous\n", bcf_seqname(args->files->readers[0].header,arec));
args->seen_seq[chr_id] = 1;
args->prev_chr = chr_id;
args->prev_pos_check = -1;
}
if ( !brec )
{
bcf_translate(args->out_hdr, args->files->readers[0].header, arec);
if ( args->nswap )
phase_update(args, args->out_hdr, arec);
bcf_update_format_int32(args->out_hdr,arec,"PS",args->phase_set,nsmpl);
bcf_write(args->out_fh, args->out_hdr, arec);
if ( arec->pos < args->prev_pos_check )
error("FIXME, disorder: %s:%d in %s vs %d written [3]\n", bcf_seqname(args->files->readers[0].header,arec), arec->pos+1,args->files->readers[0].fname, args->prev_pos_check+1);
args->prev_pos_check = arec->pos;
return;
}
int m = args->mbuf;
args->nbuf += 2;
hts_expand(bcf1_t*,args->nbuf,args->mbuf,args->buf);
for (i=m; i<args->mbuf; i++)
args->buf[i] = bcf_init1();
SWAP(bcf1_t*, args->files->readers[0].buffer[0], args->buf[args->nbuf-2]);
SWAP(bcf1_t*, args->files->readers[1].buffer[0], args->buf[args->nbuf-1]);
}
static void concat(args_t *args)
{
int i;
if ( args->phased_concat ) // phased concat
{
// keep only two open files at a time
while ( args->ifname < args->nfnames )
{
int new_file = 0;
while ( args->files->nreaders < 2 && args->ifname < args->nfnames )
{
if ( !bcf_sr_add_reader(args->files,args->fnames[args->ifname]) ) error("Failed to open %s: %s\n", args->fnames[args->ifname],bcf_sr_strerror(args->files->errnum));
new_file = 1;
args->ifname++;
if ( args->start_pos[args->ifname-1]==-1 ) break; // new chromosome, start with only one file open
if ( args->ifname < args->nfnames && args->start_pos[args->ifname]==-1 ) break; // next file starts on a different chromosome
}
// is there a line from the previous run? Seek the newly opened reader to that position
int seek_pos = -1;
int seek_chr = -1;
if ( bcf_sr_has_line(args->files,0) )
{
bcf1_t *line = bcf_sr_get_line(args->files,0);
bcf_sr_seek(args->files, bcf_seqname(args->files->readers[0].header,line), line->pos);
seek_pos = line->pos;
seek_chr = bcf_hdr_name2id(args->out_hdr, bcf_seqname(args->files->readers[0].header,line));
}
else if ( new_file )
bcf_sr_seek(args->files,NULL,0); // set to start
int nret;
while ( (nret = bcf_sr_next_line(args->files)) )
{
if ( !bcf_sr_has_line(args->files,0) ) // no input from the first reader
{
// We are assuming that there is a perfect overlap, sites which are not present in both files are dropped
if ( ! bcf_sr_region_done(args->files,0) ) continue;
phased_flush(args);
bcf_sr_remove_reader(args->files, 0);
}
// Get a line to learn about current position
for (i=0; i<args->files->nreaders; i++)
if ( bcf_sr_has_line(args->files,i) ) break;
bcf1_t *line = bcf_sr_get_line(args->files,i);
// This can happen after bcf_sr_seek: indel may start before the coordinate which we seek to.
if ( seek_chr>=0 && seek_pos>line->pos && seek_chr==bcf_hdr_name2id(args->out_hdr, bcf_seqname(args->files->readers[i].header,line)) ) continue;
seek_pos = seek_chr = -1;
// Check if the position overlaps with the next, yet unopened, reader
int must_seek = 0;
while ( args->ifname < args->nfnames && args->start_pos[args->ifname]!=-1 && line->pos >= args->start_pos[args->ifname] )
{
must_seek = 1;
if ( !bcf_sr_add_reader(args->files,args->fnames[args->ifname]) ) error("Failed to open %s: %s\n", args->fnames[args->ifname],bcf_sr_strerror(args->files->errnum));
args->ifname++;
}
if ( must_seek )
{
bcf_sr_seek(args->files, bcf_seqname(args->files->readers[i].header,line), line->pos);
seek_pos = line->pos;
seek_chr = bcf_hdr_name2id(args->out_hdr, bcf_seqname(args->files->readers[i].header,line));
continue;
}
// We are assuming that there is a perfect overlap, sites which are not present in both files are dropped
if ( args->files->nreaders>1 && !bcf_sr_has_line(args->files,1) && !bcf_sr_region_done(args->files,1) ) continue;
phased_push(args, bcf_sr_get_line(args->files,0), args->files->nreaders>1 ? bcf_sr_get_line(args->files,1) : NULL);
}
if ( args->files->nreaders )
{
phased_flush(args);
while ( args->files->nreaders )
bcf_sr_remove_reader(args->files, 0);
}
}
}
else if ( args->files ) // combining overlapping files, using synced reader
{
while ( bcf_sr_next_line(args->files) )
{
for (i=0; i<args->files->nreaders; i++)
{
bcf1_t *line = bcf_sr_get_line(args->files,i);
if ( !line ) continue;
bcf_translate(args->out_hdr, args->files->readers[i].header, line);
bcf_write1(args->out_fh, args->out_hdr, line);
if ( args->remove_dups ) break;
}
}
}
else // concatenating
{
kstring_t tmp = {0,0,0};
int prev_chr_id = -1, prev_pos;
bcf1_t *line = bcf_init();
for (i=0; i<args->nfnames; i++)
{
htsFile *fp = hts_open(args->fnames[i], "r"); if ( !fp ) error("Failed to open: %s\n", args->fnames[i]);
bcf_hdr_t *hdr = bcf_hdr_read(fp); if ( !hdr ) error("Failed to parse header: %s\n", args->fnames[i]);
if ( !fp->is_bin && args->output_type&FT_VCF )
{
line->max_unpack = BCF_UN_STR;
// if VCF is on both input and output, avoid VCF to BCF conversion
while ( hts_getline(fp, KS_SEP_LINE, &fp->line) >=0 )
{
char *str = fp->line.s;
while ( *str && *str!='\t' ) str++;
tmp.l = 0;
kputsn(fp->line.s,str-fp->line.s,&tmp);
int chr_id = bcf_hdr_name2id(args->out_hdr, tmp.s);
if ( chr_id<0 ) error("The sequence \"%s\" not defined in the header: %s\n(Quick workaround: index the file.)\n", tmp.s, args->fnames[i]);
if ( prev_chr_id!=chr_id )
{
prev_pos = -1;
if ( args->seen_seq[chr_id] )
error("\nThe chromosome block %s is not contiguous, consider running with -a.\n", tmp.s);
}
char *end;
int pos = strtol(str+1,&end,10) - 1;
if ( end==str+1 ) error("Could not parse line: %s\n", fp->line.s);
if ( prev_pos > pos )
error("The chromosome block %s is not sorted, consider running with -a.\n", tmp.s);
args->seen_seq[chr_id] = 1;
prev_chr_id = chr_id;
if ( vcf_write_line(args->out_fh, &fp->line)!=0 ) error("Failed to write %d bytes\n", fp->line.l);
}
}
else
{
// BCF conversion is required
line->max_unpack = 0;
while ( bcf_read(fp, hdr, line)==0 )
{
bcf_translate(args->out_hdr, hdr, line);
if ( prev_chr_id!=line->rid )
{
prev_pos = -1;
if ( args->seen_seq[line->rid] )
error("\nThe chromosome block %s is not contiguous, consider running with -a.\n", bcf_seqname(args->out_hdr, line));
}
if ( prev_pos > line->pos )
error("The chromosome block %s is not sorted, consider running with -a.\n", bcf_seqname(args->out_hdr, line));
args->seen_seq[line->rid] = 1;
prev_chr_id = line->rid;
if ( bcf_write(args->out_fh, args->out_hdr, line)!=0 ) error("Failed to write\n");
}
}
bcf_hdr_destroy(hdr);
hts_close(fp);
}
bcf_destroy(line);
free(tmp.s);
}
}
static void usage(args_t *args)
{
fprintf(stderr, "\n");
fprintf(stderr, "About: Concatenate or combine VCF/BCF files. All source files must have the same sample\n");
fprintf(stderr, " columns appearing in the same order. Can be used, for example, to\n");
fprintf(stderr, " concatenate chromosome VCFs into one VCF, or combine a SNP VCF and an indel\n");
fprintf(stderr, " VCF into one. The input files must be sorted by chr and position. The files\n");
fprintf(stderr, " must be given in the correct order to produce sorted VCF on output unless\n");
fprintf(stderr, " the -a, --allow-overlaps option is specified.\n");
fprintf(stderr, "Usage: bcftools concat [options] <A.vcf.gz> [<B.vcf.gz> [...]]\n");
fprintf(stderr, "\n");
fprintf(stderr, "Options:\n");
fprintf(stderr, " -a, --allow-overlaps First coordinate of the next file can precede last record of the current file.\n");
fprintf(stderr, " -D, --remove-duplicates Output only once records present in multiple files.\n");
fprintf(stderr, " -f, --file-list <file> Read the list of files from a file.\n");
fprintf(stderr, " -l, --ligate Ligate phased VCFs by matching phase at overlapping haplotypes\n");
fprintf(stderr, " -q, --min-PQ <int> Break phase set if phasing quality is lower than <int> [30]\n");
fprintf(stderr, " -o, --output <file> Write output to a file [standard output]\n");
fprintf(stderr, " -O, --output-type <b|u|z|v> b: compressed BCF, u: uncompressed BCF, z: compressed VCF, v: uncompressed VCF [v]\n");
fprintf(stderr, " -r, --regions <region> restrict to comma-separated list of regions\n");
fprintf(stderr, " -R, --regions-file <file> restrict to regions listed in a file\n");
fprintf(stderr, "\n");
exit(1);
}
int main_vcfconcat(int argc, char *argv[])
{
int c;
args_t *args = (args_t*) calloc(1,sizeof(args_t));
args->argc = argc; args->argv = argv;
args->output_fname = "-";
args->output_type = FT_VCF;
args->min_PQ = 30;
static struct option loptions[] =
{
{"regions",1,0,'r'},
{"regions-file",1,0,'R'},
{"remove-duplicates",0,0,'D'},
{"allow-overlaps",0,0,'a'},
{"ligate",0,0,'l'},
{"output",1,0,'o'},
{"output-type",1,0,'O'},
{"file-list",1,0,'f'},
{"min-PQ",1,0,'q'},
{0,0,0,0}
};
while ((c = getopt_long(argc, argv, "h:?o:O:f:alq:Dr:R:",loptions,NULL)) >= 0)
{
switch (c) {
case 'r': args->regions_list = optarg; break;
case 'R': args->regions_list = optarg; args->regions_is_file = 1; break;
case 'D': args->remove_dups = 1; break;
case 'q': args->min_PQ = atoi(optarg); break;
case 'a': args->allow_overlaps = 1; break;
case 'l': args->phased_concat = 1; break;
case 'f': args->file_list = optarg; break;
case 'o': args->output_fname = optarg; break;
case 'O':
switch (optarg[0]) {
case 'b': args->output_type = FT_BCF_GZ; break;
case 'u': args->output_type = FT_BCF; break;
case 'z': args->output_type = FT_VCF_GZ; break;
case 'v': args->output_type = FT_VCF; break;
default: error("The output type \"%s\" not recognised\n", optarg);
};
break;
case 'h':
case '?': usage(args); break;
default: error("Unknown argument: %s\n", optarg);
}
}
while ( optind<argc )
{
args->nfnames++;
args->fnames = (char **)realloc(args->fnames,sizeof(char*)*args->nfnames);
args->fnames[args->nfnames-1] = strdup(argv[optind]);
optind++;
}
if ( args->allow_overlaps && args->phased_concat ) args->allow_overlaps = 0;
if ( args->file_list )
{
if ( args->nfnames ) error("Cannot combine -l with file names on command line.\n");
args->fnames = hts_readlines(args->file_list, &args->nfnames);
}
if ( !args->nfnames ) usage(args);
if ( args->remove_dups && !args->allow_overlaps ) error("The -D option is supported only with -a\n");
if ( args->regions_list && !args->allow_overlaps ) error("The -r/-R option is supported only with -a\n");
init_data(args);
concat(args);
destroy_data(args);
free(args);
return 0;
}